1. Manufa: Wannan hanya tana nufin samar da daidaitaccen tsari don ayyukan aseptic da kariya daga dakunan bakararre.
2. Iyakar aikace-aikace: dakin gwaje-gwaje na nazarin halittu
3. Mutum Mai Alhaki: Gwajin Kula da QC
4.Ma'anar: Babu
5. Kariyar tsaro
Yi aiki mai ƙarfi na aseptic don hana gurɓataccen ƙwayoyin cuta;ya kamata masu aiki su kashe fitilar UV kafin su shiga daki maras kyau.
6.Tsarin aiki
6.1.Yakamata a samar da dakin bakararre tare da dakin aiki mara kyau da kuma dakin ajiya.Tsaftar dakin aiki mara kyau ya kamata ya kai aji 10000. Ya kamata a kiyaye zafin jiki na cikin gida a 20-24 ° C kuma a kiyaye zafi a 45-60%.Tsaftar benci mai tsafta yakamata ya kai aji 100.
6.2.Ya kamata a tsaftace ɗakin da bakararre, kuma an haramta shi sosai a tara tarkace don hana kamuwa da cuta.
6.3.Tsayayyen hana gurɓata duk kayan aikin haifuwa da kafofin watsa labarai na al'ada.Wadanda suka gurbata su daina amfani da su.
6.4.Ya kamata a sanye da ɗakin bakararre tare da masu kashe ƙwayoyin cuta masu aiki, kamar 5% crsol solution, 70% barasa, maganin 0.1% chlormethionine, da sauransu.
6.5.Yakamata a rika bakar dakin da bakararre akai-akai kuma a tsaftace shi tare da maganin da ya dace don tabbatar da cewa tsaftar dakin bakararre ya cika bukatu.
6.6.Duk kayan kida, kayan kida, jita-jita da sauran abubuwan da ake buƙatar shigar da su cikin ɗaki mara kyau yakamata a nannade su sosai kuma a ba su haifuwa ta hanyoyin da suka dace.
6.7.Kafin shiga cikin daki mai bakararre, ma'aikata dole ne su wanke hannayensu da sabulu ko maganin kashe kwayoyin cuta, sannan su canza zuwa tufafin aiki na musamman, takalma, huluna, abin rufe fuska da safar hannu a cikin dakin ajiya (ko kuma sake goge hannayensu da 70% ethanol) kafin su shiga dakin bakararre.Yi ayyuka a ɗakin ƙwayoyin cuta.
6.8.Kafin amfani da dakin bakararre, dole ne a kunna fitilar ultraviolet a cikin dakin bakararre don haskakawa da haifuwa fiye da mintuna 30, kuma dole ne a kunna benci mai tsabta don hura iska a lokaci guda.Bayan an gama aikin, ya kamata a tsaftace ɗakin bakararre cikin lokaci sannan kuma a bace ta hasken ultraviolet na minti 20.
6.9.Kafin dubawa, marufin waje na samfurin gwajin ya kamata a kiyaye shi daidai kuma kada a buɗe shi don hana kamuwa da cuta.Kafin dubawa, yi amfani da ƙwallan auduga 70% na barasa don lalata saman waje.
6.10.A yayin kowane aiki, ya kamata a yi mummunan iko don bincika amincin aikin aseptic.
6.11.Lokacin shan ruwan kwayan cuta, dole ne a yi amfani da ƙwallon tsotsa don sha.Kada ka taɓa bambaro kai tsaye da bakinka.
6.12.Dole ne a haifuwar allurar rigakafin ta hanyar harshen wuta kafin da bayan kowace amfani.Bayan sanyaya, ana iya yin allurar al'adar.
6.13.Ya kamata a jika bambaro, bututun gwaji, kayan abinci na petri da sauran kayan aikin da ke ɗauke da ruwan kwayan cuta a cikin bokitin haifuwa mai ɗauke da maganin Lysol 5% don rigakafin, sannan a fitar da shi a wanke bayan sa'o'i 24.
6.14.Idan akwai ruwan kwayan cuta da ya zube akan tebur ko kasa, nan da nan sai a zuba sinadarin carbolic acid 5% ko 3% Lysol a wurin da ya gurbata na akalla mintuna 30 kafin a yi maganinsa.Lokacin da tufafin aiki da huluna suka gurɓata da ruwan ƙwayar cuta, ya kamata a cire su nan da nan kuma a wanke su bayan haifuwar tururi mai tsanani.
6.15.Duk abubuwan da ke ɗauke da ƙwayoyin cuta masu rai dole ne a shafe su kafin a wanke su ƙarƙashin famfo.An haramta shi sosai don ƙazantar da magudanar ruwa.
6.16.Ya kamata a duba adadin mazaunan da ke cikin ɗakin da bakararre ya kamata a duba kowane wata.Tare da buɗe benci mai tsafta, ɗauki jita-jita maras kyau na petri tare da diamita na ciki na 90 mm, kuma a yi masa allura kusan ml 15 na matsakaicin al'adun agar na gina jiki wanda aka narke kuma aka sanyaya shi zuwa kusan 45 ° C.Bayan ƙarfafawa, sanya shi juye a 30 zuwa 35 Incubate na tsawon awanni 48 a cikin incubator ℃.Bayan tabbatar da haihuwa, ɗauki faranti 3 zuwa 5 kuma sanya su a hagu, tsakiya da dama na matsayi na aiki.Bayan an bude murfin da fallasa su na tsawon minti 30, sanya su a kife a cikin 30 zuwa 35 ° C incubator na sa'o'i 48 kuma a fitar da su.bincika.Matsakaicin adadin ƙwayoyin cuta daban-daban akan faranti a cikin yanki mai tsabta na aji 100 ba zai wuce mallaka 1 ba, kuma matsakaicin adadin a cikin aji mai tsabta na 10000 ba zai wuce yankuna 3 ba.Idan an wuce iyaka, ɗakin bakararre ya kamata a shafe shi sosai har sai an sake duba abubuwan da ake buƙata.
7. Koma zuwa babin (Hanyar Binciken Haihuwa) a cikin "Hanyoyin Kula da Tsaftar Magunguna" da "Ka'idojin Ayyukan Sinanci don Binciken Magunguna".
8. Sashen Rarraba: Sashen Gudanar da Inganci
Jagoran fasaha mai tsafta:
Bayan samun yanayi mara kyau da kayan bakararre, dole ne mu kula da yanayi mara kyau don yin nazarin takamaiman sanannun ƙwayoyin cuta ko amfani da ayyukansu.In ba haka ba, ƙwayoyin cuta daban-daban daga waje suna iya haɗuwa cikin sauƙi cikin sauƙi. Lamarin cakuɗewar ƙwayoyin cuta marasa amfani daga waje ana kiranta ƙwayoyin cuta masu cutarwa a cikin ƙwayoyin cuta.Hana gurɓatawa hanya ce mai mahimmanci a cikin aikin ƙwayoyin cuta.Cikakkun haifuwa a gefe guda da rigakafin gurɓatacce a daya bangaren abubuwa biyu ne na fasahar aseptic.Bugu da ƙari, dole ne mu hana ƙananan ƙwayoyin cuta da ake nazari, musamman ma ƙwayoyin cuta ko kwayoyin halitta waɗanda ba su wanzu a cikin yanayi, daga tserewa daga kwantena na gwaji zuwa yanayin waje.Don waɗannan dalilai, a cikin ƙananan ƙwayoyin cuta, akwai matakan da yawa.
Dakin bakararre yawanci ƙaramin ɗaki ne wanda aka kafa musamman a dakin gwaje-gwajen ƙwayoyin cuta.Ana iya gina shi da zanen gado da gilashi.Yankin bai kamata ya zama babba ba, kusan murabba'in mita 4-5, kuma tsayin ya kamata ya zama kusan mita 2.5.Yakamata a kafa dakin ma'auni a wajen daki mara kyau.Ƙofar ɗakin ajiya da ƙofar ɗakin bakararre bai kamata su fuskanci alkibla ɗaya ba don hana iska daga shigo da ƙwayoyin cuta iri-iri.Duk ɗakin da bakararre da ɗakin matsewa dole ne su kasance marasa iska.Dole ne kayan aikin samun iska na cikin gida su sami na'urorin tace iska.Dole ne bene da bangon ɗakin bakararre ya zama santsi, da wahalar ɗaukar datti da sauƙin tsaftacewa.Ya kamata farfajiyar aikin ta kasance daidai.Dukan ɗaki maras kyau da ɗakin buffer suna sanye da fitilun ultraviolet.Fitilar ultraviolet a cikin dakin bakararre yana da nisan mita 1 daga saman aikin.Ya kamata ma'aikatan da ke shiga dakin bakararre su sa tufafi da bakararre da huluna.
A halin yanzu, dakunan bakararre galibi suna wanzuwa a masana'antar microbiology, yayin da manyan dakunan gwaje-gwaje na amfani da benci mai tsabta.Babban aikin benci mai tsafta shine yin amfani da na'urar kwararar iska ta laminar don cire ƙananan ƙura daban-daban ciki har da ƙananan ƙwayoyin cuta a saman aiki.Na'urar lantarki tana ba da damar iska ta ratsa ta cikin tace hepa sannan ta shiga filin aiki, ta yadda a ko da yaushe filin aikin ya kasance a karkashin kulawar iska mara kyau.Bugu da ƙari, akwai labulen iska mai sauri a gefen kusa da waje don hana iskan ƙwayoyin cuta na waje shiga.
A wuraren da ke da mawuyacin yanayi, ana iya amfani da akwatunan bakararre na katako a maimakon benci mai tsabta.Akwatin bakararre yana da tsari mai sauƙi kuma yana da sauƙin motsawa.Akwai ramuka biyu a gaban akwatin, waɗanda aka toshe su ta hanyar ƙofofin turawa lokacin da ba a aiki ba.Kuna iya mika hannun ku yayin aiki.Babban ɓangaren gaba yana sanye da gilashi don sauƙaƙe aikin ciki.Akwai fitilar ultraviolet a cikin akwatin, kuma ana iya shigar da kayan aiki da ƙwayoyin cuta ta cikin ƙaramin kofa a gefe.
Dabarun aiki na Aseptic a halin yanzu ba kawai suna taka muhimmiyar rawa a cikin bincike da aikace-aikace na ƙwayoyin cuta ba, har ma ana amfani da su sosai a yawancin fasahar halittu.Misali, fasahar transgenic, fasahar antibody monoclonal, da sauransu.
Lokacin aikawa: Maris-06-2024