1. Manufa: Wannan hanya ce da nufin bayar da daidaitaccen tsarin don Aseeptic Ayyuka da kariya na ɗakuna.
2. Dokar aikace-aikacen: binciken gwajin na halitta
3. Mutumin da ke da alhaki: Mai kula da QC
4.Da babu
5. Takaddar Tsaro
Tsananin yin ayyukan ta'addanci don hana gurbataccen ƙwayar cuta; Masu aiki ya kamata su kashe fitilar UV kafin shigar da ɗakin bakararre.
6.ROCORA
6.1. Bakararre dakin ya kamata a sanye take da dakin aiki na bakararre da dakin buffer. Tsabtacewar ɗakin aikin bakararre ya isa aji 10000. Ya kamata a kula da zafin jiki na ciki a 20-24 ° C da zafi a cikin%. Da tsabta daga cikin benci mai tsabta yakamata ya kai aji 100.
6.2. Ya kamata a tsabtace ɗakin bakararre, kuma an haramta shi don tara tarkace don hana gurbatawa.
6.3. A tsananin hana gurbata dukkanin kayan masarufi da kafofin watsa labarai na al'adu. Wadanda suka gurbata ya kamata su daina amfani da su.
6.4. Starole dakin ya kamata a sanye shi da maganin tattarawa, kamar 5% cresol bayani, 70% barasa, 0.1% bayani bayani, da sauransu.
6.5. Kada a tsabtace dakin bakararre a kai a kai kuma a tsabtace shi da abin da ya dace don tabbatar da cewa tsabtace dakin bakararre ya hadu da bukatun.
6.6. Duk kayan aiki, kayan kida, jita-jita da sauran abubuwa waɗanda ke buƙatar shigo da su cikin ɗakin bakararre ya kamata a rufe su kuma an haife shi ta hanyar hanyoyin da suka dace.
6.7. Kafin shigar da ɗaki na bakararre, ma'aikata dole ne su wanke hannayensu da sabulu na musamman ko kuma maye gurbinsu da safofin hannu. Yi aiki a cikin wani kwatannin kwayan cuta.
6.8. Kafin amfani da ɗakin bakararre, fitilar ulilioet a cikin ɗakin bakararre dole ne a kunna ta don methadia da minti 30, da kuma benci na da tsabta dole ne a kunna kan sama da iska a lokaci guda. Bayan an kammala aikin, dakin bakararre ya kamata a tsabtace ta cikin lokaci sannan a haife ta hanyar hasken Ultawoltelet na minti 20.
6.9. Kafin bincika, abin da ke ƙarƙashin samfurin samfurin ya kamata a kiyaye cikin kwanciyar hankali kuma dole ne a buɗe don hana gurbatawa. Kafin bincika, yi amfani da 70% barasa auduga auduga don lalata sararin samaniya.
6.10. A kowane aiki, ya kamata a yi sarrafawa mara kyau don bincika amincin Aseptic.
6.11. A lokacin da sha ruwa na kwayan cuta, dole ne ka yi amfani da ball na ƙwanƙwasa don ɗaukar shi. Kada ku taɓa bambaro kai tsaye tare da bakinku.
6.12. Dole ne a taɓa allura ta inoculation ta hanyar harshen wuta kafin kuma bayan kowane amfani. Bayan sanyaya, za a iya samar da al'adun.
6.13. Strestaukar man shanu, bututun gwaji, kayan abinci na petri da sauran kayan amfani da ke ɗauke da ruwa mai ɗauke da ƙwayar cuta 5% don kamuwa da cuta, kuma cire shi bayan awa 24.
6.14. Idan akwai ƙwayoyin cuta na ƙwayoyin cuta akan tebur ko bene, ya kamata ka zuba 5% maganin acid na acid ko 3% Lysol akan yankin da aka gurbata na akalla minti 30 kafin a kula da shi. A lokacin da tufafi da huluna da aka gurbata su da ruwan kwayan cuta, ya kamata a cire su nan da nan kuma a wanke su bayan heam steam steam sturi.
6.15. Duk abubuwan da ke ɗauke da ƙwayoyin cuta na rayuwa dole ne a rushe su kafin a shafa shi a ƙarƙashin Matsa. An haramta shi sosai don ƙazantar da lambatu.
6.16. Yawan mulkin mallaka a cikin dakin bakararre ya kamata a bincika kowane wata. Tare da benci na tsabta, ɗauki da yawa daga petri na bakararre petri tare da diamita na ciki na matsakaici na nazarin abinci mai gina jiki da aka narke da sanyaya wa yau da kullun 45 ° C. Bayan haka, sanya shi juye a 30 zuwa 35 incubate na 48 hours a cikin wani ℃ incubator. Bayan tabbatar da farfadowa, ɗaukar faranti 3 zuwa 5 kuma sanya su a hagu, tsakiya da kuma dama na matsayin aiki. After opening the cover and exposing them for 30 minutes, place them upside down in a 30 to 35°C incubator for 48 hours and take them out. bincika. Matsakaicin adadin ƙwayoyin cuta a cikin farantin a cikin yanki mai tsabta 100 na ƙasa ba zai wuce ƙasa-ƙasa ba, kuma matsakaicin ɗakin a cikin ɗakunan da ke cikin 10000. Idan an kashe iyaka, ya kamata a gurbata dakin bakararre sosai har sai anyi amfani da bincike game da maimaita buƙatun.
7. Koyar da sura (hanyar Makon dubawa) a cikin "Hanyar Tsarin Murfuritiens" da kuma "ayyukan ingantattun kwayoyi don binciken kwayoyi".
8. Sashin Rarrabawa: Sashen Gudanarwa
Tsabtace Room Fasaha Jagora:
Bayan samun ƙauyuka na bakararre da kayan bakararre, dole ne mu kula da yanayin bakararre don yin nazarin takamaiman microorganism sanannu ko amfani da ayyukan su. In ba haka ba, ƙwayoyin cuta daban-daban daga waje na iya haɗuwa da sauƙi a ciki. An sami sabon abu na haɗuwa da ƙwayoyin cuta a cikin ilimin halittar cuta. Hawaye gurɓatawa shine dabara mai mahimmanci a cikin aikin ƙwayoyin cuta. Cikakken haifuwa a gefe ɗaya da rigakafin gurbata a kan ɗayan bangarori biyu ne na yanayin tashin hankali. Bugu da kari, dole ne mu hana microsganisms a karkashin bincike, musamman pathogenic microorganisms wanda ba sa wanzuwa cikin yanayi, daga tserewa daga kwantena na gwaji zuwa yanayin da muke ciki. Don waɗannan dalilai, a cikin halittar halittu, akwai matakan da yawa.
Sterile dakin galibi dakin da aka kafa musamman a cikin dakin gwaje-gwaje na microbi. Za a iya gina tare da zanen gado da gilashi. Yankin kada ya yi yawa, kusan murabba'in mita 4-5, kuma tsayin ya kamata ya zama kusan mita 2.5. Ya kamata a kafa ɗakin buffer a waje bakar fata. Dofar da Room Room da ƙofar ɗakin bakararre kada ta fuskance wannan hanyar don hana iska daga kawowar ƙwayoyin cuta. Dukansu bakararre da dakin buffer dole ne su kasance airtight. Kayan kayan iska na cikin gida dole ne su sami kayan tuki. Bambon da ganuwar dakin bakararre dole ne ta zama santsi, da wuya ajin datti da sauƙin tsaftacewa. Babban aikin ya zama matakin. Dukansu bakararre dakin da dakin buffer suna sanye da fitilun ultraviolet. Haske na ultorioet a cikin dakin bakararre sune mita 1 daga saman wurin. Ma'aikatan sun shiga dakin bakararre yakamata ya sa sutura haifuwa da huluna.
A halin yanzu, ɗakunan bakararre suna wanzu a cikin masana'antar masana'antu, yayin da manyan gwaje-gwaje suke amfani da benci. Babban aikin benci ne don amfani da na'urar kwararar iska don cire ƙaramin raya wanda ya haɗa da ƙananan ƙwayoyin cuta a kan farfajiya. Na'urar Lantarki tana ba da iska ta wuce iska ta wuce ta tace Heppa sannan sannan shigar da wani aiki farfajiya, don a adana sararin samaniya a ƙarƙashin ikon da iska mai gudana. Haka kuma, akwai labulen iska mai sauri a gefe zuwa waje don hana ƙwayoyin ƙwayoyin cuta na waje daga shiga.
A cikin wurare tare da mawuyacin yanayi, ana iya amfani da kwalaye na katako a maimakon benci. Akwatin bakararre yana da tsari mai sauƙi kuma yana da sauƙi don motsawa. Akwai ramuka guda biyu a gaban akwatin, waɗanda tura ƙofofin cire ƙofofin lokacin da ba a aiki ba. Kuna iya tsawaita hannuwanku a yayin aiki. A ɓangaren ɓangaren gaba yana sanye da gilashi don sauƙaƙe aiki na ciki. Akwai wani kwanon fitila a cikin kwandon shara, da kayan amfani da ƙwayoyin cuta za'a iya sa su ta hanyar kofa a gefe.
Hanyoyin aiki na ASeptic a halin yanzu ba kawai suna taka rawar gani a cikin binciken bincike da aikace-aikacen ba, amma ana amfani da su da yawa a cikin yawancin ilimin halittar halitta. Misali, fasaha ta Transgenic, fasaha ta monoclonal, da sauransu.
Lokacin Post: Mar-06-2024